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Abstract
Lactic acid bacteria are known for their potential use in food preservation. Wild-type fruits of Ecuadorian Amazon tropical rainforest were used to isolate and identify new bacteriocinogenic lactic acid species. Among several isolates, Lactobacillus plantarum strain Gt4 (GenBank No. KY041689) showing highly inhibitory capacity was further characterized. Using agar-well diffusion assay, the crude-extract (CE) and precipitated bacteriocin (PP) derived from Gt4 strain displayed elevated antimicrobial activity towards several pathogenic bacteria. Its active principle was proteinaceous since the bacteriocin was inhibited by proteolytic enzymes and showed grater thermostability. Adsorption of the bacteriocin to pathogenic cells was increased when treated with EDTA, NaCl, Tween 20, at acidic pH and temperature of 37°C and 45°C. A decrease in adsorption was registered when cells were treated with SDS. Based on Tricine SDS-PAGE the estimated molecular weight was 10 kDa showing bactericidal mode of action.
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