revista accb, artículos académicos, artículos. biología, ciencias, ACCB, biologicas

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PDF. Pag, 113-119 ()

Keywords

Antibacterial
gastric cancer
minimum inhibitory concentration
inhibition. (DeCS/MESH) Antibacteriano
cáncer gástrico
concentración mínima inhibitoria
inhibición (DeCS/MESH).

How to Cite

Valdés, L. C. ., Obando, C. D. ., & Pazos, A. . (2021). nhibitory effect of seed polyphenolic fractions of Persea americana var. Hass on the Helicobacter pylori growth. REVISTA DE LA ASOCIACION COLOMBIANA DE CIENCIAS BIOLOGICAS, 1(33), 113–119. https://doi.org/10.47499/revistaaccb.v1i33.241

Abstract

Introduction: Gastric cancer (GC) is the main cause of cancer death in Colombia and of multifactorial etiology, associated with genetic factors of the host, environmental factors and the oncopathogen Helicobacter pylori (H. pylori) as the main risk factor of the disease. The avocado (Persea americana var. Hass, (P. americana)) has a high content of polyphenols, with bactericidal, antioxidant, anti-urease, and anti-inflammatory activity, which in turn limit changes in the gastric environment that favor colonization bacteria and subsequent triggering of the precancerous cascade. Objective: To evaluate the minimum inhibitory concentration of polyphenolic fractions of P. americana seed on the in vitro growth of H pylori. Results: The evaluation of the minimum inhibitory concentration (MIC) of the three fractions obtained from the P. americana seed in H. pylori culture in vitro, showed that fractions one and two inhibited bacterial growth at 3000 ppm, while fraction 3 at 1500 ppm, P <0.05. Conclusion: Three polyphenolic biofractions of the P. americana seed were found, mainly constituted by procyanidins, with in vitro antibacterial activity of Helicobacter pylori, however, fraction three, with a higher molecular weight, significantly inhibited growth at a MIC of 1500 ppm, compared to fractions one and two.

 

https://doi.org/10.47499/revistaaccb.v1i33.241

References

Otero Regino, W. (2008). Cáncer gástrico en Colombia: un diagnóstico tardío que amerita el compromiso del Estado. Rev. colomb. Gastroenterol, 23(4): 302- 304.

Internacional Agency for Research on Cancer: IARC (2018). Cancer Today: GLOBOCAN 2018- Top cancer percountry, estimated number of deaths in 2018, both sexes, all ages. Recuperado de. http://gco.iarc.fr/today.

Correa, P. (2011). Cáncer gástrico: una enfermedad infecciosa. Rev. Colomb. Cirug. 26(2), 111-117.

Coussens, L. M., & Werb, Z. (2002). Inflammation and cancer. Nature, 420(6917): 860-867.

https://doi.org/10.1038/nature01322

Figueroa, M., Cortés, A., Pazos, Á., & Bravo, L. E. (2012). Sensibilidad in vitro a amoxicilina y claritromicina de Helicobacter pylori obtenido de biopsias gástricas de pacientes en zona de bajo riesgo para cáncer gástrico. Biomédica; 32(1): 32-42.

Urgesi, R., Cianci, R., & Riccioni, M. E. (2012). Update on triple therapy for eradication of Helicobacter pylori: current status of the art. Clin Exp Gastroenterol, 5:151-157

https://doi.org/10.2147/CEG.S25416

Megraud, F. (2004). H pylori antibiotic resistance: prevalence, importance, and advances in testing. Gut, 53(9): 1374-1384.

https://doi.org/10.1136/gut.2003.022111

De Francesco, Vincenzo, V., Giorgio, F., Hassan, C., Manes, G., et al. (2010). Worldwide H. pylori antibiotic resistance: a systematic. J Gastrointestin Liver Dis, 19(4): 409-414.

Pastene, E.; Hebel, S.; García, A. (2012). Polifenoles con efecto anti-Helicobacter pylori: fuentes de obtención y su potencial utilización en fitomedicamentos, nutracéuticos y alimentos funcionales. Rev Farmacol Chile, 5: 35-50.

Malbrán, C. (2012). Metodo de determinación de sensibilidad antimicrobiana por dilucion [MIC testing]. M07-A9; 32(2). 32 (2). Disponible en: https://iris.paho.org/bitstream/handle/10665.2/51470/v43e652019.pdf?sequence=1&isAllowed=y

Alarcón, T., Baquero, M., Domingo, D., Lopéz, M., & Royo, G. (2004). diagnóstico microbiológico de la infección por Helicobacter pylori. En L. Manuel, Procedimientos en microbiología clinica (págs. 75-105). Madrid: SEIMC.

Wong, Chun-Foong; Yeo, Joshua Yi; Gan, Samuel Ken-En (2016). APD Colony Counter App: Using Watershed algorithm for improved colony counting. Nat. Methods Appl. Notes, 1-3. https://oar.a-star.edu.sg/storage/o/o13no50vx3/775-an9774.pdf

Chávez, F., Aranda, M., García, A., & Pastene, E. (2011). Los polifenoles antioxidantes extraídos del epicarpio de Palta (Persea americana var. Hass) inhiben la ureasa de Helicobacter pylori. B Latinoam Caribe PL. 10(3): 265-280.

Romero, M., Freire, J. Pastene, E., García, A., Aranda, M., & González, C. (2019). Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro. Rev. Bras. Farmacog., 29: 325-332.

https://doi.org/10.1016/j.bjp.2019.03.002

Murali, M. R., Naveen, S. V., Son, C. G., & Raghavendran, H. R. B. (2014). Current knowledge on alleviating Helicobacter pylori infections through the use of some commonly known natural products: bench to bedside. Integrat. Med. Resear., 3(3): 111-118.

https://doi.org/10.1016/j.imr.2014.04.001

Basante, J., Cruz, S., Osorio, C., & Hurtado, N. (2016). Obtención, fraccionamiento y determinación de la composición de extractos polifenólicos de aguacate (Persea americana Mill). In: Congreso Iberoamericano de Productos Naturales, 2016, Bogotá, Colombia. Disponible en: http://sired.udenar.edu.co/id/eprint/3651

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