revista accb, artículos académicos, artículos. biología, ciencias, ACCB, biologicas

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PDF. Pag, 56-67 (Español (España))

Keywords

crucífera
dilución
placa
medios de cultivo
fitopatología
micopatógenos
rizosfera crucifera
silver solution
culture media
phytopathology
mycopathogens
rhizosphere

How to Cite

Ocampo Arias , M. C. ., Ocampo Madrid, N. A. ., Gómez Rondón, L. D. ., Lora Suarez, F. ., & Soto Agudelo, R. . (2023). Identification of phytopathogenic fungi associated with Brassica oleracea var. Capitata in the Armenia city, Quindío. REVISTA DE LA ASOCIACION COLOMBIANA DE CIENCIAS BIOLOGICAS, 1(35), 56–67. https://doi.org/10.47499/revistaaccb.v1i35.288

Abstract

Introduction. Phytopathogenic fungi represent the main economic losses worldwide in different types of crops, including vegetables such as Brassica Oleracea var. capitata. Objectives. Identify the possible phytopathogenic fungi associated with Brassica, the signs and symptoms they may cause, their possible transmission mechanism, and to qualitatively compare their growth in different culture media. Materials and methods. Leaves with and without symptoms were isolated, together with soil samples to elaborate serial dilutions. As for microscopy, the reproductive parts of the fungi were visualized for taxonomic classification. Results. A total of 17 morphotypes were isolated up to genus level, being Botrytis and Fusarium, the genera found with the highest presence in asymptomatic and symptomatic leaf samples. For soil samples, the most frequent genus was Penicillium. Conclusions. Of the three media evaluated, Sabouraud’s extract agar showed the best results for the growth of most fungi. Probable transmission mechanisms were established for each of the fungi found in association with B. oleracea var. capitata and Botrytis sp. was established as the possible pathogen causing the symptoms observed in the samples.

https://doi.org/10.47499/revistaaccb.v1i35.288

References

Rozano, V., Quiroz, C., Acosta, J., Pimentel, L., Quiñones, E. (2005). Hortalizas, las llaves de la energía. RDU. 6(9), 2-30. En https://www.revista.unam.mx/vol.6/num9/art88/sep_art88.pdf

Vargas-Rincón, C., Sánchez-León, G., Jiménez, P. (2014). La producción de metabolitos secundarios en la familia Brassicaceae. Rev. Fac Cien Básicas, 9(2), 282-305. https://doi.org/10.18359/rfcb.388

https://doi.org/10.18359/rfcb.388

Lv, H., Fang, Z., Yang, L., Zhang, Y., Wang, Q., Liu, Y., et al. (2014). Mapping and analysis of a novel candidate Fusarium wilt resistance gene FOC1 in Brassica oleracea. BMC Genomics 15, 1094. https://doi.org/10.1186/1471-2164-15-1094

https://doi.org/10.1186/1471-2164-15-1094

Gerszberg, A., Hnatuszko-Konka, K., Kowalczyk, T. (2015). In vitro regeneration of eight cultivars of Brassica oleracea var. capitata. In Vitro Cell Dev Biol-Plant 51, 80-87. https://doi.org/10.1007/s11627-014-9648-7

https://doi.org/10.1007/s11627-014-9648-7

Poveda, J., Velasco, P., Sacristán, S. (2022). Fungal endophytes of Brassicaceae: Molecular interactions and crop benefits. Front Plant Sci., 13. https://doi.org/10.3389/fpls.2022.932288

https://doi.org/10.3389/fpls.2022.932288

Davies, C., Wohlgemuth, F., Young, T., Violet, J., Dickinson, M., Sanders, J., et al, (2021). Evolving challenges and strategies for fungal control in the food supply chain. Fungal Biol Rev., 36, 15-26. https://doi.org/10.1016/j.fbr.2021.01.003

https://doi.org/10.1016/j.fbr.2021.01.003

Koike, S., Gladders, P., Paulus, A. (2007). Vegetable diseases. A colour handbook. London, UK: Manson Publishing. Pp 450.

Srivastava, M., Gupta, S., Saxena, A., & Shittu, L. (2011). A review of occurrence of fungal pathogens on significant brassicaceous vegetable crops and their control measures. Asian J Agric Sci., 3(2): 70-79. https://maxwellsci.com/print/ajas/v3-70-79.pdf

Castaño Zapata, J. (2015). Principios básicos de hongos fitopatógenos. Manizales-Caldas: ed.. Universidad de Caldas. Pp 83-99. Disponible en http://www.ebooks7-24.com.crai.referencistas.com/?il=16631

https://doi.org/10.2307/j.ctv1tqcwkn

Agrios, G. N. A. (2005). Plant diseases causes by fungi. En Plant Pathology (5.a ed., pp. 385-614): ed. Elsevier Disponible en https://doi.org/10.1016/C2009-0-02037-6

https://doi.org/10.1016/C2009-0-02037-6

Rodríguez, C. T. (1999). Biodiversidad del relicto "Cedro Rosado" de la Universidad del Quindío. Biología y Educación. 9:17-18.

Muñoz, V., Cisterna, V., France, A. (2020) Aislamiento de microorganismos fitopatógenos. Chillan: Boletín INIA. 77-91. Consultado octubre 26, 2022, en: https://hdl.handle.net/20.500.14001/67165

Monzón, A. Rodríguez Tudela, J. L. (2000). Infecciones causadas por el género fusarium. Control Calidad SEIMC. https://seimc.org/contenidos/ccs/revisionestematicas/micologia/fusarium.pdf

Piontelli, E. (2012). El complejo Pseudallescheria/Scedosporium y taxas afines en la rutina del laboratorio micológico clínico. Boletín Micológico, 27(2). https://doi.org/10.22370/bolmicol.2012.27.2.885

https://doi.org/10.22370/bolmicol.2012.27.2.885

Cepero, M., Restrepo, S., Franco-Molano, A., Cardenas, M., Vargas, N. (2012). Biología de Hongos. Bogotá. Universidad de los Andes: Ed. Ediciones Uniandes.

Cruz-Lachica, I. (2017). Identification of mucoralean fungi causing soft rot in papaya (Carica papaya L.) fruit in Mexico. Rev Mex fitopatol, 35, 3. En https://www.smf.org.mx/rmf/ojs/index.php/RMF/article/view/75

https://doi.org/10.18781/R.MEX.FIT.1611-3

Boonmee, S., Wanasinghe, N., Calabon, S., Huanraluek, N., Chandrasiri, U., Jones, G., et al. (2021). Fungal diversity notes 1387-1511: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Divers, 111(1), 1-335. https://doi.org/10.1007/s13225-021-00489-3

https://doi.org/10.1007/s13225-021-00489-3

Khan, M. A., & Khan, A. M. (2021). Diversity and distribution of soil fungi isolated from the cabbage (Brassica Oleracea var. Capitata) rhizosphere. Plant Arch, 21(1). https://doi.org/10.51470/plantarchives.2021.v21.no1.033

https://doi.org/10.51470/PLANTARCHIVES.2021.v21.no1.033

Alam, S., Sakamoto, K., & Inubushi, K. (2011). Biocontrol efficiency of Fusarium wilt diseases by a root-colonizing fungus Penicillium sp. J Soil Sci Plant Nutr., 57:2, 204-212, https://doi.org/10.1080/00380768.2011.564996

https://doi.org/10.1080/00380768.2011.564996

Sisti, M., Amagliani, G., & Brandi, G. (2003). Antifungal activity of Brassica oleracea var. botrytis fresh aqueous juice. Fitoterapia. 74,453-458. doi:10.1016/s0367-326x(03)00108-4

https://doi.org/10.1016/S0367-326X(03)00108-4

Bensch, K., Groenewald, J., Z., Meijer, M., Dijksterhuis, J., Jurjević, Ž., Andersen, B., et al. (2018). Cladosporium species in indoor environments. Stud Mycol. 89, 177-301. En doi: 10.1016/j.simyco.2018.03.002

https://doi.org/10.1016/j.simyco.2018.03.002

Archana, J., P, Nasare., M, Bhowl. (2014) Fungal Diversity in Vegetables, Int. J. Life Sci. Res. Issue 2, 25-26. Disponible en, https://www.researchgate.net/publication/279033262_Fungal_Diversity_in_Vegetables

Mookherjee, A., Bera, P., Mitra, A., Maiti, M. K. (2018). Characterization and Synergistic Effect of Antifungal Volatile Organic Compounds Emitted by the Geotrichum candidum PF005, an Endophytic Fungus from the Eggplant. Microb Ecol. 75, 647-661. En https://doi.org/10.1007/s00248-017-1065-0

https://doi.org/10.1007/s00248-017-1065-0

Saini, T., Kumar, D. & Gupta, S. (2022). First report of cauliflower anthracnose caused by Colletotrichum gigasporum in India. New Dis. Rep. 46, 12121. En https://doi.org/10.1002/ndr2.12121

https://doi.org/10.1002/ndr2.12121

Pacheco-Cano, R., Salcedo-Hernández, R., López-Meza, J., Bideshi, D., Barboza-Corona, J. (2018). Antimicrobial activity of broccoli (Brassica oleracea var. italica) cultivar Avenger against pathogenic bacteria, phytopathogenic filamentous fungi and yeast. J Appl Microbiol, 124: 126-135. https://doi.org/10.1111/jam.13629

https://doi.org/10.1111/jam.13629

Moya, H. J. (2012). Manejo fitosanitario del cultivo de hortalizas. Bogotá-Cundinamarca: ed. Instituto Colombiano Agropecuario. Disponible en https://www.ica.gov.co/getattachment/bb883b42-80da-4ae5-851f-4db05edf581b/Manejo-fitosanitario-del-cultivo-de-hortalizas.aspx

Bolívar-Anillo, H., Abdellah, E., da Silva, G., Izquierdo-Bueno, I., Moraga, J. y dos Santos, G. (2021)

Endophytic Fungal Community Associated with Colombian Plants. En Rosa, L. (2021). Rosa, L. H. (Ed.). (2021). Neotropical Endophytic Fungi. Belo Horizonte-Minas Gerais: ed.Springer. Pp 93-108. Disponible en https://doi.org/10.1007/978-3-030-53506-3_5

https://doi.org/10.1007/978-3-030-53506-3_5

Jayawardena, R., Bhunjun, C., Hyde, K., Gentekaki, E., Eleni, Itthayakorn, P.(2021). Colletotrichum: lifestyles, biology, morpho-species, species complexes and accepted species. Mycosphere 12(1),519-669. Doi 10.5943/mycosphere/12/1/7

https://doi.org/10.5943/mycosphere/12/1/7

Rupp, S., Weber, W., Rieger, D., Detzel, P., Hahn, M. (2017). Spread of Botrytis cinerea Strains with Multiple Fungicide Resistance in German Horticulture. Front Microbiol., 7, 2075. https://doi.org/10.3389/fmicb.2016.02075

https://doi.org/10.3389/fmicb.2016.02075

Li, H., Chen, Y., Zhang, Z., Li, B., Qin, G., Tian, S. (2018). Pathogenic mechanisms and control strategies of Botrytis cinerea causing post-harvest decay in fruits and vegetables. Food Qual Saf., 2, 111-119.https://doi.org/10.1093/fqsafe/fyy016

https://doi.org/10.1093/fqsafe/fyy016

Williamson, B., Tudzynski, B., Van kan, J. (2007). Botrytis cinerea: the cause of grey mould disease Mol. Plant Pathol., 8(5), 561-580. https://doi.org/10.1111/j.1364-3703.2007.00417.x

https://doi.org/10.1111/j.1364-3703.2007.00417.x

Van Kan, L., Shaw, M. (2014). Botrytis species: relentless necrotrophic thugs or endophytes gone rogue? Mol Plant Pathol., 15(9), 957-961. https://doi.org/10.1111/mpp.12148

https://doi.org/10.1111/mpp.12148

Arie, T. (2019). Fusarium diseases of cultivated plants, control, diagnosis, and molecular and genetic studies. J Pestic Sci., 44(4), 275-281. https://doi.org/10.1584/jpestics.J19-03

https://doi.org/10.1584/jpestics.J19-03

Moretti, L., Busso-Lopes, A., Tararam, A., Moraes, R., Muraosa, Y., Mikami, Y., et al. (2018). Airborne transmission of invasive fusariosis in patients with hematologic malignancies. PloS one, 13(4).https://doi.org/10.1371/journal.pone.0196426

https://doi.org/10.1371/journal.pone.0196426

Wallon, T., Sauvageau, A., Van der Heyden, H. (2020). Detection and Quantification of Rhizoctonia solani and Rhizoctonia solani AG1-IB Causing the Bottom Rot of Lettuce in Tissues and Soils by Multiplex qPCR. Plants, 10(1), 57. https://doi.org/10.3390/plants10010057

https://doi.org/10.3390/plants10010057

Senapati, M., Tiwari, A., Sharma, N., Chandra, P., Bashyal, M., Ellur, K., et al. (2022). Rhizoctonia solani Kühn Pathophysiology: Status and Prospects of Sheath Blight Disease Management in Rice. Front Plant Sci., 13 (881116). https://doi.org/10.3389/fpls.2022.881116

https://doi.org/10.3389/fpls.2022.881116

Budge, E., Shaw, W., Lambourne, C., Jennings, P., Clayburn, R., Boonham, N. et al. (2009). Characterization and origin of infection of Rhizoctonia solani associated with Brassica oleracea crops in the UK. Plant Pathol, 58,1059-1070. https://doi.org/10.1111/j.1365-3059.2009.02138.x

https://doi.org/10.1111/j.1365-3059.2009.02138.x

Benli, İ., Türkkan, M., Özer, G., Erper, I. (2021). First report of Rhizoctonia solani AG 1-IB causing root and stem rot of kale (Brassica oleracea var. acephala) in Turkey. J Plant Pathol 103, 663. https://doi.org/10.1007/s42161-021-00750-3

https://doi.org/10.1007/s42161-021-00750-3

Park, Y., Oyaizu, H., Okada, G., Takahashi, M. (2002). Screening of fungal antagonists against yellows of cabbage caused by Fusarium oxysporum f. sp. conglutinans. Mycoscience, 43(6), 447-451. https://doi.org/10.1007/s102670200064

https://doi.org/10.1007/S102670200064

Sarkar, D., Rakshit, A. (2021). Bio-priming in combination with mineral fertilizer improves nutritional quality and yield of red cabbage under Middle Gangetic Plains, India. Sci Hortic., 283 (110075). https://doi.org/10.1016/j.scienta.2021.110075

https://doi.org/10.1016/j.scienta.2021.110075

Orozco, M., Manzo, G., Guzmán, S., Farías, Javier., Timmer, L. (2004). Crecimiento y Cambios Morfológicos de Colletotrichum acutatum Simmonds, Agente Causal de la Antracnosis del Limón Mexicano (Citrus aurantifolia Christm. Swingle) Incubado en diferentes medios. Rev- mex. fitopatol. 22(3), 423-428.

Gulmez, O., Baris, O. (2022). Fungal Growth and Pathology. Intech Open. http://dx.doi.org/10.5772/intechopen.103109

https://doi.org/10.5772/intechopen.103109

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