In vitro preservation of Cyrtopodium schargellii using the minimal growth technique
Supplementary Files
Keywords
bosque seco tropical
explantes
medios de cultivo
orquídea
osmorreguladores Biotechnology
tropical dry forest
explants
growing media
orchid
osmoregulators
How to Cite
Abstract
Introduction: Cyrtopodium schargellii, known in certain areas as ‘lluvia dorada’, is an orchid native to Central America, distributed in countries such as Venezuela and Colombia. It is possible to find this orchid in Colombia in the department of Córdoba and other surrounding areas. Objective: The objetive of this research was to establish a viable and effective protocol for the in vitro conservation of the orchid C. schargellii; Materials and methods: for this purpose, seedlings of this species previously germinated in vitro were used, and then explants were taken and sown in culture media supplemented with 100% MS salts and two osmoregulators (mannitol and sorbitol) in four treatments for each substance: T1 (0.5%), T2 (1%), T3 (1.5%) and T4 (2%). In addition, a control was prepared (T0, without osmoregulators and with 100% MS salts). Results: In the case of mannitol, shoot and root formation was observed, accompanied by chlorosis and death of some explants, while in the case of sorbitol, little shoot and root formation was observed, but all explants survive. Conclusion: In conclusion, the in vitro preservation protocol given by 0.5% sorbitol was the best treatment for the preservation of this orchid.
References
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